The course deals with the application of advanced methods in single- and multi-component spectrophotometric and spectrofluorimetric analysis of pharmaceutical compounds. Chemical derivatization and functional group analysis using colorimetric methods will be discussed. It will include investigation of deviations from Lambert-Beer’s law, stray light effects, and relative photometric concentration error. Suitable UV-light absorbing systems will be utilized to demonstrate the mathematical methods used in multi-component spectrophotometric analysis of pharmaceuticals. First- and second-derivative absorption curves methods to illustrate the use of derivative UV-spectroscopy in quantitation analysis.